Oxford United central defender Phil Gilchrist has been forced to retire through injury.
He has been unable to recover from a persistent knee injury, which has troubled him for the past nine months.
"It's been a hard decision. One of the toughest of my life," the 34-year-old told Oxford's website.
"Every footballer wants to play for as long as possible but it's got to the stage where the injury is restricting what I can do on the pitch."
Gilchrist added: "It is also causing problems for a few days after."
Gilchrist, who joined Oxford for a second spell in June 2006, had been limited to just three appearances for the U's this season.
His career, in which he started over 500 senior games, also included spells with Hartlepool, Leicester City, West Bromwich Albion and Rotherham United.