Holden was injured in a challenge with Netherland's Nigel de Jong
Bolton midfielder Stuart Holden has been ruled out for six weeks after suffering a fracture to his right leg.
Holden was injured during the United States' 2-1 friendly defeat by the Netherlands on Wednesday.
The 24-year-old, who joined Wanderers in January, started Bolton's last two matches - against Wolves and Tottenham.
And Bolton boss Owen Coyle told the club website: "In both games we saw his quality so it's a blow, the important thing is he returns bigger and better."
After leaving Houston Dynamo, Holden signed for Bolton in January on a deal until the end of the season - and Coyle confirmed on Thursday he would be taking up an option to extend the deal until the end of next season.
Coyle is also hopeful the injury will not damage Holden's prospects of playing in the World Cup, with the United States playing England in their opening group game on 12 June.
This page is best viewed in an up-to-date web browser with style sheets (CSS) enabled. While you will be able to view the content of this page in your current browser, you will not be able to get the full visual experience. Please consider upgrading your browser software or enabling style sheets (CSS) if you are able to do so.