The Czech Republic have suffered their second late injury withdrawal after Tomas Galasek withdrew from the Euro 2008 qualifier with Wales on Saturday.
Czech midfielder Tomas Galasek has a stomach muscle problem
The Nuremberg midfielder, 34, has a stomach muscle strain, joining defender Martin Jiranek on the sidelines.
On Wednesday manager Karel Bruckner was forced to call up Tomas Hubschman for Jiranek, but has opted not to summon a replacement for Galasek.
The Czechs were already without Zdenek Grygera for the match in Cardiff.
Spartak Moscow's Jiranek has a chronic inflammation of his Achilles tendon so Hubschman, who plays for Ukraine's Shakhtar Donetsk, replaces him.
Ajax defender Grygera, who is set to move to Juventus, injured his left knee in last March's match with Cyprus.
Bruckner called up Udinese's Tomas Zapotocny to replace him for Saturday's match at the Millennium Stadium.
Zapotocny's club colleague midfielder Tomas Sivok has been picked for the first time since September 2005.
Captain Tomas Rosicky, the Arsenal midfielder, is included despite having four wisdom teeth removed last week, as is Chelsea goalkeeper Petr Cech.
"It was nothing pleasant," said Rosicky. "Everybody who has a toothache knows what I am talking about.
"But it all went on without any complications and I did restart training quite soon afterwards."
Bruckner has also selected rookie striker Lubos Pecka, who is the top scorer in the Czech league this season with 16 goals for Mlada Boleslav, in his 19-man squad.
The Czech Republic, Germany and the Republic of Ireland top Group D with 13 points apiece, although Ireland have played seven matches, the Czechs six and Germany five.
Czech Republic squad:
Goalkeepers: Petr Cech (Chelsea), Jaromir Blazek (Sparta Prague).
Defenders: Marek Jankulovski (AC Milan), Tomas Hubschman (Shakhtar Donetsk), Radoslav Kovac (Spartak Moscow), David Rozehnal (Paris Saint-Germain), Tomas Ujfalusi (Fiorentina), Tomas Zapotocny (Udinese).
Midfielders: David Jarolim (Hamburger SV), Marek Matejovsky (Mlada Boleslav), Jaroslav Plasil (Monaco), Jan Polak (FC Nuremberg), Tomas Rosicky (Arsenal), Tomas Sivok (Udinese).
Forwards: Milan Baros (Lyon), Jan Koller (Monaco), Marek Kulic (Sparta Prague), Lubos Pecka (Mlada Boleslav).