Craig Bellamy has failed in his bid to get a court date changed that falls during Wales' Euro 2008 qualifying match against Cyprus on 11 October.
Bellamy could attend court in the day and then play for Wales
The Liverpool striker, 27, is accused of the common assault of two women in a Cardiff nightclub last February.
However, Bellamy could still play for Wales despite the date falling on the second day of his trial in Cardiff.
The match at the Millennium Stadium is in the evening, so Bellamy could still possibly attend court and then play.
The Football Association of Wales has indicated it has no problems with Bellamy's participation in the Cyprus match, although the ultimate decision will be down to Wales manager John Toshack.
Wales play Slovakia, also in Cardiff, on 7 October and so Bellamy will be able to attend the bulk of Wales' training and preparation ahead of the back-to-back matches.
If Craig were unable to train it would make playing difficult, but not impossible
An FAW spokesperson said: "Craig would still be picked for the squad because the match is part of a double header.
"If Craig were unable to train it would make playing difficult, but not impossible.
"He is a key player, but obviously there are some things that are outside our hands."
The court case is expected to last three days and Bellamy has denied the charges.
The player's solicitor, Matthew de Maid, had tried to have the trial at Cardiff magistrates' court brought forward to avoid a clash with Wales matches.
But district judge Gerwyn Watkins on Friday confirmed that the dates earmarked will remain unchanged.
Bellamy is alleged to have assaulted Holly Smith and Sophie Palmer at the No 10 nightclub in Cardiff on 5 February.
The player was due to stand trial at the end of August but it had to be put back as some prosecution witnesses were unavailable.
A second man, Franklin Lynch, 52, of Glan-y-Mor, Barry, south Wales, also denies common assault on Sophie Palmer.