For City, the focus switches to Sunday's lunchtime kick-off against Newcastle in the Premier League and they will hope to pick up where they left off against champions Chelsea last weekend.
The hosts arrived high on confidence following that victory and despite making four changes to their starting XI, boss Roberto Mancini still managed to send out a team a worth £132m.
Emmanuel Adebayor was handed a rare opportunity to partner Carlos Tevez in attack, while Patrick Vieira lined up against his former club, Johnson occupied the right wing and summer signing Jerome Boateng got his first City start.
Mancini, a boyhood Juve fan, admitted the alterations were made with half an eye on Newcastle and they seemed to disrupt his side, who got off to disjointed start.
There seemed a lethargy about their passing and possession was relinquished too easily. The visitors, by contrast, looked hungry and sharp on and off the ball, harrying their opponents into mistakes and getting forward en masse.
Only three minutes were on the clock when Iaquinta saw a near-post drive saved by Joe Hart after Mohamed Sissoko robbed Gareth Barry in midfield and, soon after, Hart did well to repel a long-range free-kick by Del Piero.
Juventus boss Luigi del Neri made five alterations to the side that beat Cagliari on Sunday but they settled into a tidy rhythm and deservedly took the lead when Claudio Marchisio found Iaquinta and the Italian forward rifled in a 30-yard strike that Hart might have kept out.
The momentum was with the two-time European champions and City were reduced to speculative efforts - Tevez, Vieira, Johnson and Boateng all sending shots from distance way off target - while the impressive Milos Krasic had a decent penalty appeal rejected.
Italy striker Iaquinta struck early for his third goal of the season
Mancini was becoming increasingly agitated on the touchline and his players eventually responded, with a tireless Tevez at the forefront of their resurgence.
Juventus seemed to be sitting on their lead - wide men Krasic and Jorge Martinez no longer making the runs which epitomised their team's early progress - and City capitalised.
They were almost on level terms when Barry glanced a Tevez cross on to the post and Johnson's follow-up was smothered by former Arsenal goalkeeper Alex Manninger.
Moments later City's pressure told as Yaya Toure dissected the Juve defence with a delicious slide-rule pass, which Johnson ran on to before easily beating Manninger.
Mancini's men carried their momentum into the second half but struggled to break down a defence featuring Italy international centre-backs Giorgio Chiellini and Leonardo Bonucci.
Barry hooked a shot over the bar and Tevez crossed for a disappointing Adebayor to head well wide, while at the other end Del Piero flashed a set-piece narrowly off target with Hart at full stretch.
A lull ensued as substitutes arrived and both sides seemed content with their point, although there was a late flurry of activity as another Del Piero free-kick rattled the bar and bounced on - but not over - the goal line.
Grateful for the let-off, City then pushed for a winner and Tevez and David Silva both came close to breaching the Juve backline - but the Italians held firm.
90:00+2:10 The referee penalises Carlos Tevez for handball. Alex Manninger takes the indirect free kick.
90:00+1:03 Mohamed Sissoko has an effort at goal from outside the penalty box missing to the wide right of the target.
87:33 Outswinging corner taken by Adam Johnson, Vincenzo Iaquinta manages to make a clearance.
84:59 Gareth Barry challenges Vincenzo Iaquinta unfairly and gives away a free kick. Shot comes in from Alessandro Del Piero from
the free kick,
83:20 Substitution James Milner on for Jerome Boateng.
75:00 Substitution Milos Krasic goes off and De Carvalho Felipe Melo comes on.
74:23 Zdenek Grygera gives away a free kick for an unfair challenge on Adam Johnson. Dedryk Boyata takes the free kick.
73:47 Booking Zdenek Grygera goes into the referee's book for unsporting behaviour.
72:41 Substitution David Silva replaces Emmanuel Adebayor.
70:57 Substitution Paolo De Ceglie goes off and Marco Motta comes on.
70:02 Free kick awarded for a foul by Carlos Tevez on Leonardo Bonucci. Leonardo Bonucci restarts play with the free kick.
69:34 Free kick awarded for a foul by Vincent Kompany on Vincenzo Iaquinta. Zdenek Grygera restarts play with the free kick.
66:43 Header from close range by Emmanuel Adebayor misses to the right of the goal.
64:55 Kolo Toure gives away a free kick for an unfair challenge on Alessandro Del Piero. Alessandro Del Piero has an effort direct
from the free kick.
63:54 Effort on goal by Gareth Barry from just inside the box goes harmlessly over the target.
62:22 Alessandro Del Piero restarts play with the free kick.
62:22 Booking The referee shows Gareth Barry a yellow card for unsporting behaviour.
62:12 Foul by Gareth Barry on Mohamed Sissoko, free kick awarded.
56:46 Adam Johnson takes a shot. Save by Alex Manninger.
54:53 Outswinging corner taken by Alessandro Del Piero from the right by-line, Header by Giorgio Chiellini from deep inside the
penalty area misses to the left of the target.
53:10 Substitution Simone Pepe joins the action as a substitute, replacing Jorge Martinez.
51:47 The assistant referee signals for offside against Emmanuel Adebayor. Alex Manninger takes the free kick.
47:30 Milos Krasic produces a cross, save made by Joe Hart.
45:47 Alessandro Del Piero takes a shot. Save by Joe Hart.
45:01 The game restarts for the second half.
45:01 Substitution Dedryk Boyata joins the action as a substitute, replacing Pablo Zabaleta.
45:00+1:03 The whistle is blown to end the first half.
44:43 Claudio Marchisio takes a shot. Joe Hart makes a save.
42:18 Inswinging corner taken by Alessandro Del Piero from the left by-line, save by Joe Hart.
41:20 Inswinging corner taken from the right by-line by Adam Johnson.
39:49 Booking The referee books Milos Krasic for unsporting behaviour.
37:34 Pablo Zabaleta takes a shot. Alex Manninger makes a save.
36:08 Gnegneri Toure Yaya provided the assist for the goal.
36:08 GOAL - Adam Johnson:Man City 1 - 1 Juventus Adam Johnson scores a goal from inside the area low into the middle of the goal. Man City 1-1 Juventus.
33:09 Headed effort from close in by Gareth Barry bounces off the post. Adam Johnson takes a shot. Save by Joe Hart. Inswinging
corner taken by Adam Johnson, Leonardo Bonucci manages to make a clearance. The referee blows for offside against Carlos Tevez.
Free kick taken by Alex Manninger.
31:11 Unfair challenge on Carlos Tevez by Zdenek Grygera results in a free kick. Carlos Tevez takes the free kick.
29:46 Free kick awarded for an unfair challenge on Milos Krasic by Pablo Zabaleta. Free kick taken by Alessandro Del Piero.
28:46 Shot from outside the box by Jerome Boateng goes wide right of the target.
25:56 Shot from just outside the box by Adam Johnson goes over the bar.
24:42 Claudio Marchisio fouled by Patrick Vieira, the ref awards a free kick. Alessandro Del Piero restarts play with the free kick.
21:03 Shot from outside the penalty box by Patrick Vieira goes wide right of the goal.
20:26 Emmanuel Adebayor is adjudged to have handled the ball. Indirect free kick taken by Claudio Marchisio.
Correction - 18:38 Carlos Tevez produces a right-footed shot from deep inside the penalty area which goes wide of the right-hand post.
18:38 Carlos Tevez produces a right-footed shot from the edge of the box and misses to the right of the goal.
16:18 Free kick awarded for a foul by Gnegneri Toure Yaya on Paolo De Ceglie. Giorgio Chiellini restarts play with the free kick.
11:30 Inswinging corner taken left-footed by Adam Johnson from the right by-line, Patrick Vieira takes a shot. Unfair challenge
on Alex Manninger by Gnegneri Toure Yaya results in a free kick. Alex Manninger takes the free kick. Inswinging corner taken
by Adam Johnson.
9:56 GOAL - Vincenzo Iaquinta:Man City 0 - 1 Juventus A goal is scored by Vincenzo Iaquinta from outside the penalty box to the bottom right corner of the goal. Man City 0-1 Juventus.
5:37 Free kick awarded for a foul by Vincent Kompany on Vincenzo Iaquinta. Alessandro Del Piero produces a shot on goal direct
from the free kick, Joe Hart makes a save.
3:35 Gnegneri Toure Yaya concedes a free kick for a foul on Jorge Martinez. Alessandro Del Piero takes the free kick.
3:02 Vincenzo Iaquinta takes a shot. Save by Joe Hart.
0:14 Paolo De Ceglie challenges Adam Johnson unfairly and gives away a free kick. Free kick taken by Jerome Boateng. Jerome Boateng
takes a shot.
0:00 The match gets underway.
Live text and data provided by The Press Association.
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