Europe's most powerful clubs - the G14 - will not back Uefa president Michel Platini's plan to give domestic cup winners a Champions League place.
FA cheif executive Brian Barwick has backed Platini's plan
"We're opposed to giving cup winners a place in the Champions League," said G14's general manager Thomas Kurth.
"We're very concerned about the quality of the competition and the damage this could cause," added Kurth.
Under the proposals, which could begin in 2009, the Premier League's top three and the FA Cup winners would qualify.
Platini's proposals would give 16 cup winners a separate qualifying route into the Champions League and an allocation of four automatic places in the lucrative group stages.
The proposed shake-up was discussed by UEFA's Professional Football Strategy Council last month but after some opposition from the European Professional Football Leagues (EPFL), Platini deferred the final vote by UEFA's executive by one month.
The EPFL, which represents leagues such as the English Premier League, Spain's La Liga and Italy's Serie A, was given until the end of October to come up with alternative proposals.
Football Association chief executive Brian Barwick has described Platini's plan as a "perfectly reasonable suggestion".
Meanwhile the G14, which represents 18 of Europe's top clubs including Liverpool, Arsenal and Manchester United, intends to expand its membership to around 50 by the end of the year.
The move is designed to ensure the G14 is not perceived as elitist as both Fifa and Uefa have failed to recognise or engage with the group on the grounds that it does not repersent the majority of clubs in Europe.
"We understand UEFA's concerns and we accept that we may have not been representative of all clubs, but we have now decided to make this change and expand," said Kurth told Reuters.
"If this is given a positive response by UEFA, then we hope this could be the first step in solving the problems we have."
The G14 has been at odds with Fifa and Uefa over issues such as the release of players for international duty and the international calendar.
An ongoing row over compensation for players injured while playing for their country has led to two court cases, one of which is pending at Europe's highest court - the European Court of Justice.