Lazio have taken the appeal against their match-fixing punishment to the Italian Olympic Committee (CONI).
Lotito has vowed to fight his two-and-a-half-year ban
After Juventus, Lazio are the second of the four clubs - including Fiorentina and AC Milan - penalised in the Italian scandal to take their appeal further.
Lazio were docked 11 points for the new season and banned from competing in Europe, despite reaching the UEFA Cup.
"We have received the appeal for Lazio Rome and their president Claudio Lotito," said the CONI spokesperson.
Lotito was banned for two and a half years for his role in the match-fixing affair but has vowed to fight that sanction.
Fiorentina, who have been kicked out of the Champions League and docked 17 points this season, have also stated their intention to appeal.
But AC Milan, deducted just eight points and allowed to keep their place in the Champions League, have not indicated a wish to appeal.
All four Italian clubs implicated in the match-fixing scandal have had their punishments cut after initial appeals to the Italian Football Federation (FIGC) sports court.
Juventus' hopes of overturning demotion to Serie B were dashed, but they now have a 17-point handicap, not 30.
Lazio and Fiorentina were both reinstated to Serie A - but with increased points deductions increased from 12 to 19 for Fiorentina, while AC Milan stay in the top flight, with their penalty cut from 15 points to eight.
The appeals and the whole scandal which has rocked Italian football has seen the domestic league season kick-off put back by two weeks from 27 August to 9-10 September.