Fifa's executive committee has unanimously opposed the Premier League's plan for overseas matches.
Scudamore has angered Blatter with plans to play matches abroad
The Premier League unveiled proposals on 7 February to host a 39th round of matches across five cities across the world from 2011.
But the plan has received a hostile reception and was quickly rejected at Friday's committee meeting in Zurich.
"This idea to play a 39th round outside the country does not work," said Fifa president Sepp Blatter.
"They would be playing 12 hours away west and east and 24 hours difference in the south.
"Even the former chairman of the FA, Mr Geoff Thompson, said we should oppose it."
The Premier League pulled out of a planned trip to see Blatter on 28 February.
A statement from the Premier League at the time said: "We will conduct further internal studies and consultation before seeking the advice of Fifa."