Wigan chairman Dave Whelan wants West Ham to face new charges over the signing of Carlos Tevez.
Tevez scored seven goals in 10 games to keep West Ham up
The Hammers were fined £5.5m for irregularities over the purchases of Tevez and Javier Mascherano but escaped a points deduction.
Wigan have vowed to support relegated Sheffield United in taking action.
"We need a new hearing because he has been registered illegally again. That should be another fine or points deduction," said Whelan.
"We cannot reverse the decision the commission came out with. It is impossible - they were appointed by the Premier League and all the clubs agreed these people would take charge of the case.
"But now we are saying he (Tevez) has been registered illegally for a second time. Who owns him? Where will this £30m be going we are told he is now going for?
"We have to clean all this up. Time is on the side of the Premier League, I have said to Sheffield United that they must get stuck into the League's bones in the next seven days.
"Sheffield United have told me they have a fantastically strong case."
Wigan, Fulham, Charlton and the Blades believe West Ham should have been docked points for acting improperly, while Middlesbrough support the action.
I feel cheated because losing to Wigan was not the root cause of our relegation
Sheff Utd chairman Kevin McCabe
Wigan chairman Dave Whelan attended a meeting on Monday over what action to take.
The Hammers were fined £5.5m but not docked points after being found guilty of acting improperly and withholding vital documentation over the ownership of Tevez and Mascherano, who is now at Liverpool.
Sheffield United chairman Kevin McCabe said he believed more Premier League clubs would join the action.
He told Radio Four's Today programme: "Most of the clubs support the injustice that has come about this season, I would hope we would get the backing of most.
"I feel cheated because yesterday's result was not the root cause of our relegation.
"It will need conversations with chairmen and directors so they understand all of the injustices, so I suspect we will be canvassing.
"If I were on the Premier League board, I would say 'we must address Sheffield United's plight that has come about through no cause of their own'.
"So they need to call their own internal meetings and decide what to do. It is their responsibility to pursue it just as much as it is ours."
Whelan has promised Sheffield United his full support
Middlesbrough chief executive Keith Lamb confirmed they had raised their concerns over the matter with the Premier League.
"We are not part of any legal action against the Premier League," said Lamb.
"However, in the interests of clarity we are one of several clubs who would like to see evidence that Tevez was correctly registered."
BBC Sport's Garry Richardson said that "two other clubs have joined in and they don't want to be named at the minute".
Latics chief Whelan says they must act quickly.
"Legally, we are being given strong advice that this can be challenged, whatever the Premier League says," he said.
"It can go as far as the House of Lords but the problem there is that it will take too long, next season will be under way by then.
"We must establish whether Tevez was registered in time to play for West Ham and whether they created a new transfer window to sign him.
"But the challenge has to be mounted very, very quickly. Monday and Tuesday are very important days and it has got to be pushed through.
"I feel sadness that Sheffield United went down and we will support them until the end."
West Ham escaped relegation on Sunday after a 1-0 win over Manchester United, with Tevez, ironically, scoring the winner.
United boss Sir Alex Ferguson said he was pleased for Hammers boss and friend Alan Curbishley but admitted he was uneasy over some of the factors relating to the London club's survival.
"I am delighted for Alan but I can't say I am too delighted for West Ham because of all the circumstances surrounding them," said Ferguson.
"I don't want get involved in it. It is not my domain and it doesn't involve Manchester United.
"But I do feel for Sheffield United and I know everyone around the country is looking at the situation with some concern."