Mark Carrington's cool finish for MK Dons helped end Colchester's 100% start to the League One season.
Jermaine Easter put the hosts ahead from the penalty spot after he had been barged over by U's skipper Pat Baldwin.
Carrington doubled the lead in the 55th minute, bursting past last defender Magnus Okuonghae to round Ben Williams and slot the ball into the empty net.
Scott Vernon headed in David Fox's pin-point cross to give the U's hope but they could not muster an equaliser.
Colchester United caretaker manager Joe Dunne told BBC Essex:
"We did our best and tried and tried but it just wasn't to be.
"We probably lacked the killer ball or final cross, but if we play like that, you're going to win more games than you lose.
"There's plenty of positives and we showed the reasons why we'll compete and be there or thereabouts at the end of the season."
Live text and stats
Saturday, 22 August 2009
Easter (pen) 22
12 Gueret yellow card
07 Gleeson yellow card
10 Johnson yellow card (Ibehre 81 yellow card)
21 Howell yellow card
26 Chadwick (Doumbe 68)
08 Easter (Baldock 85)
03 Beevers (Maybury 28)
11 Hackney (Perkins 63)
29 Vernon (Thomas 79)
Ref: StroudAtt: 8,633
Attempts on target
Attempts off target
90:00+5:23 The game is over, as the referee blows his whistle.
90:00+3:03 Free kick awarded for an unfair challenge on Luke Howell by Marc Tierney. Direct free kick taken by Luke Howell.
90:00+2:03 Handball decision goes against Kevin Lisbie. Free kick taken by Willy Gueret.
90:00+1:09 Substitution Mathias Doumbe is brought on as a substitute for Luke Chadwick.
89:41 Unfair challenge on Jason Puncheon by Marc Tierney results in a free kick. Jason Puncheon takes the direct free kick.
89:05 The referee blows for offside against Jason Puncheon. Ben Williams restarts play with the free kick.
88:30 Corner taken by Jason Puncheon.
87:40 Magnus Okuonghae challenges Jabo Ibehre unfairly and gives away a free kick. Direct free kick taken by Stephen Gleeson.
Correction - 86:17 Headed effort from deep inside the penalty area by Kevin Lisbie misses to the left of the goal.
86:17 Header by Kevin Lisbie from deep inside the penalty area misses to the right of the target.
85:44 Unfair challenge on Marc Tierney by Luke Howell results in a free kick. David Perkins takes the direct free kick.
84:47 Substitution Jermaine Easter leaves the field to be replaced by Sam Baldock.
84:34 Shot from deep inside the area by Kemal Izzet clears the crossbar.
83:45 Jason Puncheon decides to take the corner short.
83:08 Jason Puncheon takes a corner.
80:21 Booking Jabo Ibehre is shown a yellow card for unsporting behaviour.
Correction - 80:06 Substitution Jabo Ibehre is brought on as a substitute for Jemal Johnson.
80:06 Substitution Jemal Johnson goes off and Jabo Ibehre comes on.
78:40 Substitution Joel Thomas on for Scott Vernon.
72:43 Shot from just outside the penalty box by David Fox misses to the left of the target.
71:44 David Fox takes the direct free kick.
71:44 Booking Stephen Gleeson is shown a yellow card.
71:33 Free kick awarded for a foul by Stephen Gleeson on David Perkins.
69:58 Ashley Vincent challenges Dan Woodards unfairly and gives away a free kick. Willy Gueret takes the free kick.
68:20 Assist on the goal came from David Fox. Corner from the right by-line taken by David Fox, Jason Puncheon manages to make a
68:20 GOAL - Scott Vernon:MK Dons 2 - 1 Colchester Scott Vernon finds the net with a headed goal from close in. Milton Keynes Dons 2-1 Colchester.
68:15 The ball is crossed by David Fox,
Correction - 67:14 Substitution Mathias Doumbe replaces Luke Chadwick.
66:29 Inswinging corner taken from the right by-line by David Fox.
65:43 Effort on goal by Ashley Vincent from 25 yards. Save by Willy Gueret.
64:37 Free kick awarded for a foul by Kemal Izzet on Jason Puncheon. Direct free kick taken by Jason Puncheon.
63:10 Ashley Vincent takes a shot. Save by Willy Gueret.
62:19 Substitution David Perkins is brought on as a substitute for Simon Hackney.
61:05 Free kick crossed by David Fox.
61:05 Booking Luke Howell is booked.
60:39 Kevin Lisbie fouled by Luke Howell, the ref awards a free kick.
59:40 Dan Woodards fouled by Kemal Izzet, the ref awards a free kick. Jason Puncheon takes the free kick.
57:06 GOAL - Mark Carrington:MK Dons 2 - 0 Colchester Mark Carrington gets on the score sheet with a goal from deep inside the penalty box low into the middle of the goal. Milton
Keynes Dons 2-0 Colchester.
55:15 The assistant referee signals for offside against Jason Puncheon. Indirect free kick taken by Ben Williams.
54:30 Shot from inside the six-yard box by Jason Puncheon goes wide right of the target.
53:44 Free kick awarded for a foul by Jason Puncheon on Kemal Izzet. David Fox takes the direct free kick.
51:28 Free kick awarded for an unfair challenge on Mark Carrington by Pat Baldwin. Direct free kick taken by Willy Gueret.
50:52 Free kick awarded for an unfair challenge on Kemal Izzet by Mark Carrington. David Fox takes the free kick.
49:36 Unfair challenge on Alan Maybury by Jemal Johnson results in a free kick. Magnus Okuonghae takes the free kick.
48:04 Booking Caution for Willy Gueret.
47:14 Corner taken by Simon Hackney from the right by-line.
46:43 Corner from the right by-line taken by David Fox, clearance made by Mark Carrington.
45:01 The second half kicks off.
45:00+4:11 The referee blows for half time.
45:00+1:26 The assistant referee signals for offside against Kemal Izzet. Willy Gueret takes the indirect free kick.
Correction - 44:56 Effort on goal by Ashley Vincent from 25 yards. Inswinging corner taken left-footed by Simon Hackney from the left by-line.
Kevin Lisbie gets a block in.
44:56 Shot from 25 yards by Ashley Vincent. Corner taken left-footed by Simon Hackney from the left by-line, blocked by Kevin Lisbie.
43:51 Corner taken by Jemal Johnson.
42:12 Jermaine Easter takes a shot. Save by Ben Williams. Shot from deep inside the area by Jermaine Easter clears the bar. Foul
by David McCracken on Scott Vernon, free kick awarded. Free kick taken by David Fox.
40:49 Effort from 25 yards by Kemal Izzet. Save by Willy Gueret.
39:14 Booking Jemal Johnson is cautioned by the ref for unsporting behaviour.
38:56 Jemal Johnson gives away a free kick for an unfair challenge on Alan Maybury. Ben Williams restarts play with the free kick.
37:52 Corner taken by Jemal Johnson from the left by-line.
37:30 Jermaine Easter takes a shot. Save by Ben Williams.
34:48 Shot from just outside the area by Ashley Vincent misses to the right of the target.
32:55 The referee blows for offside against Jason Puncheon. Ben Williams takes the indirect free kick.
31:31 Outswinging corner taken by David Fox.
30:57 Kevin Lisbie fouled by Mark Carrington, the ref awards a free kick. Free kick crossed right-footed by David Fox, Sol Davis
makes a clearance.
30:22 Effort from inside the area by Jermaine Easter misses to the right of the target.
29:25 Free kick awarded for a foul by David Fox on Jason Puncheon. Direct free kick taken by Jason Puncheon.
29:02 Simon Hackney takes a inswinging corner.
28:04 Ashley Vincent fouled by Jemal Johnson, the ref awards a free kick. Free kick crossed by Simon Hackney, save by Willy Gueret.
27:14 Substitution Alan Maybury on for Lee Beevers.
27:14 Jermaine Easter gives away a free kick for an unfair challenge on Magnus Okuonghae. Free kick taken by Ben Williams.
23:53 Lee Beevers fouled by Jemal Johnson, the ref awards a free kick. Ben Williams takes the direct free kick.
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