AFC Wimbledon enjoyed a 1-0 win over FC United of Manchester on Saturday as the two fan-run clubs met at Kingsmeadow.
Wimbledon's Shane Smeltz takes on FC United's Rob Nugent
Trialist Ricci Crace got the only goal on 56 minutes for the Ryman Premier side, in front of a crowd of 3,301.
FC United manager Karl Marginson said: "I'm proud of all the lads - I knew they could play, but now I know they can handle a big occasion."
Dons boss Dave Anderson added: "They were an unknown quantity, but it turned out to be a really useful workout."
AFC Wimbledon were formed in 2002 after Wimbledon FC's move to Milton Keynes was sanctioned by an FA commission - while FC United were created in June 2005 by disaffected Manchester United fans following Malcolm Glazer's takeover.