Guinea coach Robert Nouzaret has been forced to replace French-born defender Kevin Constant with Mamadou Dioulde Bah in his squad for the Africa Cup of Nations finals.
Robert Nouzaret has been forced to change his Guinea squad
The Guinea Football Federation said the Toulouse defender had not been given permission by Fifa to play for the country at the tournament in Ghana.
There was some better news for Guinea though as they thrashed fellow Nations Cup finalists Sudan 6-0 in a warm up game in Spain on Friday.
Souleymane Youla from French Ligue 1 strugglers Lille struck four goals and Ukraine-based fellow striker Ismael Bangoura and midfielder Victor Correa from French lower league club Cherbourg scored once each.
"Fifa have told us he does not qualify to play in this competition," Fode Capi Camara, secretary general of the Guinea Football Federation said.
"With the permission of the player we sent a dossier to Fifa to prove the Guinean origins of his mother to allow him to play for the national team.
"Unfortunately this investigation will not been completed in time."
The 20-year-old Constant has already been capped by the west Africans in a friendly match against Senegal in October.
Replacement Bah is from French club Racing Strasbourg but has yet to make a first team appearance.
The 19-year-old is also uncapped at international level.
Guinea play hosts Ghana in the opening game of the Nations Cup in Accra on 20 January, before games against Morocco and Namibia.
Kemoko Camara (unattached), Naby Diarso (Satellite FC), Naby Yattara (RACS Couillet, Belgium)
Dianbobo Balde (Celtic, Scotland), Habib Jean Balde (Reims, France), Alseny Camara (Rodez, France), Ibrahima Camara (Le Mans, France), Mamadou Dioulde Bah (Strasbourg, France), Mohamed Alimou Diallo (Sivasspor, Turkey), Daouda Jabi (Trabzonspor, Turkey), Oumar Kalabane (Manisaspor, Turkey), Kamil Zayatte (Young Boys Berne, Switzerland)
Mohamed Cisse (Bursaspor, Turkey), Pascal Feindouno (St Etienne, France), Samuel Johnson (Ismaili, Egypt), Mohamed Sacko (Etoile du Sahel, Tunisia), Naby Soumah (CS Sfaxien, Tunisia), Kanfory Sylla (Sivasspor, Turkey)
Ismael Bangoura (Dynamo Kiev, Ukraine), Karamoko Cisse (Verona. Itlay), Victor Correa (Cherbourg, France), Fode Mansare (Toulouse, France), Souleymane Youla (Lille, France)