All-rounder Darren Sammy and batsman Travis Dowlin have been added to the Stanford Superstars line-up for the series involving England in Antigua.
The duo will replace injured all-rounder Dwayne Bravo and exciting West Indies opener Xavier Marshall.
No official reason has been given for the withdrawal of Marshall, though the West Indies Cricket Board told Cricinfo he had failed a drugs test.
Tonito Willett, on a training camp with Marshall, also tested positive.
The series culminates with the $20m winner-takes-all game between the All-Stars and England on 1 November.
Four players in the squad - Lennox Cush, Chad Hampson, Lindon James and Lionel Baker - have no international experience.
November's match will be the first of five annual fixtures between the two sides, following an agreement between billionaire businessman Sir Allen Stanford, who has staged two 20/20 tournaments in the Caribbean, and the England and Wales Cricket Board.
Each player on the winning side will pocket £500,000 ($1m) - a total of £5.5m - with a further £1m to be divided between their coach and the rest of the squad.
The remaining £3.5m will be split between the ECB and the West Indies Cricket Board.
Stanford, who has described Test cricket as "boring", believes Twenty20 can become the biggest team sport in the world and hopes the matches against England will help popularise it in the American market.
Revised Stanford Allstars squad: Lionel Baker, Sulieman Benn, Shivnarine Chanderpaul, Lennox Cush, Travis Dowlin, Rayad Emrit, Andre Fletcher, Chris Gayle, Chad Hampson, Lindon James, Sylvester Joseph, Dave Mohammed, Kieron Pollard, Daren Powell, Darren Sammy, Ramnaresh Sarwan, Jerome Taylor.
Stanford Super Series schedule:
25 Oct: Stanford Super Stars v Trinidad & Tobago
26 Oct: England v Twenty20 Champions
27 Oct: Trinidad & Tobago v Twenty20 champions
28 Oct: England v Trinidad & Tobago
29 Oct: Stanford Super Stars v Twenty20 champions
30 Oct: Rest day
31 Oct: Legends Beach Cricket match
1 Nov: Stanford Super Stars v England