Yousuf (right) has led Pakistan in four one-day internationals
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Mohammad Yousuf will captain Pakistan in the Champions Trophy after Younis Khan ruled himself out of contention.
Younis was expected to deputise for Inzamam-ul-Haq who has been banned for four games, but told a news conference he refused to be a "dummy captain".
He could now face disciplinary action by the PCB although he will continue as a player for the tournament.
"We don't think this is the way to do things," said cricket board director of operations Saleem Altaf.
"If he had any problems he should have discussed it with us first.
"We will talk to Younis about his behaviour."
All-rounder Abdul Razzaq will be the vice-captain for the event, which begins in India on Saturday.
Pakistan's opening match is on 17 October against a team from the qualifying round, probably either Sri Lanka or West Indies.
Younis, normally Inzamam's vice-captain, was asked to step up while Inzamam served a ban for his part in the forfeited Oval Test.
The batsman should have held a full news conference with coach Bob Woolmer prior to the team's departure for India but instead he said one sentence and left.
The PCB on Thursday held an emergency briefing at its headquarters in Lahore between chairman Shaharyar Khan and other officials, including Inzamam.
Yousuf, known as Yousuf Youhana before his conversion to Islam, has previously led Pakistan in four one-day internationals, winning two.