Collingwood is England's second-most capped one-day international player
England will rest Paul Collingwood for the next three one-day internationals and James Anderson for the next two in their series against Australia.
England are 3-0 down in the seven-match series but with the Champions Trophy and Test series in South Africa to come this year the pair have been rested.
"They need a rest," said coach Andy Flower, "they are picking up niggles."
Graham Onions and Joe Denly could play in Saturday's game at Lord's and Stuart Broad and Adil Rashid may return.
Onions, who has yet to feature in a one-day international, was added to the squad ahead of the third match to bolster England's bowling options.
"I think it is an exciting opportunity for a couple of other guys to step into those roles and do them well," added Flower.
I want to go out and get a big one and win games for England
"We see these guys in the dressing room every day and we make the judgment on who needs the rest and who doesn't.
"They [Collingwood and Anderson] have been in the spotlight a long time and they need a break.
"We also want them to come back into the squad before we get to the Champions Trophy."
Ricky Ponting returns to lead Australia at Lord's after being granted a fortnight rest period following the 2-1 Ashes defeat.
Cameron White, who has scored 200 runs in three innings deputising for Ponting, including a maiden ODI hundred at the Rose Bowl on Wednesday, is expected to switch to number seven, with Mike Hussey likely to miss out.
"I might have to take someone down at training to get myself back into the side," said Ponting.
"Cameron's played really well. I've been impressed with how he's played in all three innings.
"I think he showed us all over the last couple of weeks he's more than capable of batting anywhere in the order.
"That's a real positive for us and makes selections for Saturday's game a little more difficult."
Kent opener Denly was named in the initial squad, but has yet to feature after injuring his knee while warming up by playing football prior to the first match in the series.
All-rounder Broad has recovered from a minor neck injury, while promising Yorkshire leg-spinner Rashid, dropped for the last two matches, could also return after England's poor display at the Rose Bowl.
England fly out to South Africa on 21 September, meaning the only chance to combat fatigue amongst the players involved in the heavy schedule following the Ashes triumph is to rest them against world champions Australia.
Anderson will join up with the squad again for the second of two one-day internationals at Trent Bridge on Thursday.
Collingwood will return for England's final match of the campaign at Chester-le-Street next Sunday.
Meanwhile, out-of-form batsman Ravi Bopara feels he has a big part to play in the future of English cricket despite a horror run of form for his country this summer.
Bopara's highest score in seven Ashes innings was 35, before he was dropped for the decider, and he is yet to hit a half-century in six one-day innings.
The Essex batsman said: "There is a lot to come from me yet. I know I can play, it is just a case of getting my game in order.
"Personally I want to go out and get a big one and win games for England.
"I don't want to get scores that just do enough to give England a chance to win, I want to go out and seal the win for England.
"That is my ambition and I am desperate to do that. Not over-desperate because that's when things can go wrong, but I want to be the main man for England."