England captain Michael Vaughan will miss Friday's game against Australia because of a torn left hamstring.
Vaughan has missed most of the last year through injury
The extent of the injury he suffered in the win over New Zealand in Hobart was revealed by a scan on Wednesday.
It is still unclear how long Vaughan will be out of action and the injury is to be reassessed in the next 48 hours.
Andrew Flintoff will lead the team in Brisbane and Ravi Bopara may make his debut, but England have also called up Mal Loye to provide extra cover.
Bopara normally bats at five for Essex in one-day cricket, so it could mean Ed Joyce reverting to the top of the order in place of Vaughan, although Loye provides an extra option.
"It's a great boost for me to be in with a chance," said 34-year-old Loye, who has been playing provincial cricket in New Zealand for Auckland.
"I got a few runs against Otago the other day and if I do play on Friday I'll feel pretty confident."
He told BBC Sport: "I'm really excited and after the Ashes series I know what to expect from the Australians. But at the end of the day performance is everything.
"The last couple of seasons I've done pretty well for Lancashire. A lot of that is down to [the staff at] Lancashire and I am very thankful for the opportunity I've been given."
606 DEBATE: Your thoughts on Vaughan's injury
Vaughan suffered the injury, described as a grade one tear, after running around from backward point and turning sharply during New Zealand's innings.
He required a runner soon after coming out to bat and made 17 as England clinched a three-wicket win - their first victory during the tour of Australia.
He was playing only his second one-day international after surgery to solve career-threatening problems on his right knee.
The Yorkshire star was forced to return home early from the winter tours of Pakistan and India in 2005/6.