England opener Marcus Trescothick has been left out of the squad for next month's ICC Champions Trophy.
Trescothick's immediate England future remains unclear
He made himself unavailable because of a "sensitive medical condition" but has insisted he still wants to tour Australia this winter.
England chairman of selectors David Graveney said: "It is a blow Marcus will not be available but we are delighted he will be for Australia."
England have until Sunday to pick their squad for the tournament in India.
Trescothick's plea to skip the Champions Trophy follows his return home from India in February when he initially blamed personal reasons then a virus for his departure.
England coach Duncan Fletcher confirmed on Wednesday that there had been talks about the 30-year-old's availability for what has been dubbed the "mini World Cup".
I am now well on course to make a full recovery and am determined to play a full part in the Ashes tour
Trescothick's medical specialists
"It's a very sensitive issue, a medical issue," said Fletcher.
Trescothick sought advice from specialists after returning home from India earlier in the year and they have now advised he rests after the conclusion of the one-day series with Pakistan.
"I was keen to take part in the ICC Champions Trophy but I do appreciate that my specialist's advice is that I must first continue the treatment devised for me," Trescothick said.
"Having come to terms with the full extent of my illness last winter, I recognise that I am now well on course to make a full recovery and I am determined to play a full part in the Ashes tour."
A spokesman for his specialists Performance Healthcare described his condition as "acquired gastrointestinal infection".
"It later became evident he was also suffering from an underlying stress-related illness," he added.
Since walking out of India, Trescothick's form has been patchy.
The 30-year-old bounced back with a century in the opening Test against Sri Lanka at Lord's but since then his average has dropped away to 19.7.
In the one-day game, he scored centuries against Sri Lanka at Headingley and Ireland in Belfast but scored just 22 runs in the latest three games against Pakistan. He was out first ball at the Rose Bowl.
His decision to opt out of the Champions Trophy throws England's one-day plans into disarray, with the 2007 World Cup looming on the horizon in March.
The ICC gave England an extension on Tuesday to name their 14 players because of continuing injury problems among key players.
All-rounder Andrew Flintoff (ankle) and pacemen Steve Harmison (back) and Darren Gough (shin) are all doubtful, while Michael Vaughan (knee) remains a long-term concern.
The defeat by Pakistan in the third match of the NatWest Series extended England's poor run to just four wins in the last 24 limited-overs matches.
England begin their Champions Trophy campaign on 15 October against India.