Andrew Strauss will lead a new-look 15-man England squad in the one-dayers against Sri Lanka after Andrew Flintoff and Michael Vaughan were ruled out.
Strauss deputised as skipper in two one-dayers in India
Five uncapped players have been called up - Tim Bresnan, Glen Chapple, Jamie Dalrymple, Ed Joyce and Alex Loudon.
Flintoff must rest his injured ankle for four weeks and Vaughan (knee) will build up his fitness at Yorkshire.
Steve Harmison is back after his shin problem but Simon Jones, Ashley Giles and James Anderson remain sidelined.
Strauss told BBC Radio Five Live: "Andrew Flintoff will be sorely missed, but too often in the past we've relied too much on him.
"Now other people have got to stand up. It's important that we learn to win without him.
"I don't view myself as the new England captain but it is a very important job for this one-day series.
"This is a very big series for us on the road to the 2007 World Cup and the selectors have given a few guys the opportunity to see what they can do."
Andrew Strauss did an excellent job captaining the team in difficult circumstances in India
The squad bears little resemblance to that which was hammered 5-1 in the one-day series in India earlier this year.
Kabir Ali, Gareth Batty, Matthew Hoggard, Matt Prior, Owais Shah and Vikram Solanki are all dropped, while Ian Blackwell is also injured.
Batsman Alastair Cook, who broke into the Test team in India and kept his place against Sri Lanka, is in line to make his one-day international debut.
Vaughan, who scored 99 in the County Championship on Wednesday, could play some part later in the series.
Chairman of selectors David Graveney said: "We do not want to take any unnecessary risks with Michael Vaughan and will wait until he is fully match-fit before considering him for selection.
"This will be a tough test for the team. Our first-choice captain is injured, his nominated replacement Andrew Flintoff is also unavailable, as are a number of senior players.
"Andrew Strauss did an excellent job captaining the team in difficult circumstances in India, securing our only win in the series and will bring his experience of leading Middlesex to the role."
The selection of Chapple at the age of 32 will raise some eyebrows, but the seamer has been in fine form for Lancashire.
He and 21-year-old Yorkshire seamer Tim Bresnan, who has pipped two fast bowlers - Tom Smith of Lancashire and Leicestershire's Stuart Broad - are also handy lower-order batsmen.
Dalrymple, 25, has been steady if not spectacular as a batsman who bowls a bit for Middlesex, while his Irish-born county team-mate Joyce qualified for England at the end of last season.
England face Ireland on Tuesday, Sri Lanka in a Twenty20 game on Thursday at the Rose Bowl, before the five-match one-day series starts next Saturday at Lord's.
England squad: Andrew Strauss (Middlesex, captain), Ian Bell (Warwickshire), Tim Bresnan (Yorkshire), Glen Chapple (Lancashire), Paul Collingwood (Durham), Alastair Cook (Essex), James Dalrymple (Middlesex), Stephen Harmison (Durham), Geraint Jones (Kent), Ed Joyce (Middlesex), Alex Loudon (Warwickshire), Sajid Mahmood (Lancashire), Kevin Pietersen (Hampshire), Liam Plunkett (Durham), Marcus Trescothick (Somerset).