CLYDESDALE BANK 40, Canterbury: Kent 249-7 beat Scotland 191-7 by 54 runs
Blake's previous best was 80 against Derbyshire at Chesterfield last summer
Scotland suffered their second CB40 defeat in 24 hours against Kent at Canterbury to all but end their hopes of qualifying for the semi-finals.
Youngster Alex Blake hit a career-best 81 not out as Kent piled up 249-7, backed by Azhar Mahmood's 44.
But, despite Tasmania skipper George Bailey top scoring for the second day running with 70, aided by Neil McCallum (45), Scotland fell short on 191-7.
Amjad Khan took 3-32 as Kent secured only their second win by 58 runs.
Blake went to the wicket with his side in a spot of bother on 108-4, a situation that quickly worsened when in-form Darren Stevens drove on the up against Ross Lyons to go for 24.
That brought together Blake, playing only his 13th List A game, and the experienced Mahmood for a sixth-wicket stand worth 102 in 24 overs that turned the course of the game.
Alex Blake told BBC Radio Kent: "It's been a while since I've got a 50 so it was nice to spend some time at the crease.
"I've been told to play straight early on to get myself in then later I would be able to play a few cross-bat shots.
"I've finished my second year at university. If I'm in the twos [Kent second team] then I'll go back to Leeds to play for university but if I'm in the first team then I will stay down."