The England and Wales Cricket Board will allow players to refer decisions to the TV umpire in the Friends Provident 50-over one-day competition.
Sussex's star bowler Mushtaq Ahmed appeals in a 2006 match
Only the captain of the fielding side and the batsman involved in the dismissal may refer a decision.
Each team is allowed two unsuccessful referrals per innings on all modes of dismissal, apart from timed out.
ECB operations chief Alan Fordham said: "We are undertaking this trial as we are committed to innovation."
He went on: "While we acknowledge the high standard of umpiring in our domestic game, we believe the opportunity to explore ways that we can achieve even more correct decisions must be taken, and this is the aim of the trial."
Nottinghamshire's Chris Read said he was not certain the initiative would be a success.
He told BBC Radio Five Live: "I am personally a little bit sceptical as to how it will work but unless you try these things you don't know.
"We can now bring in those little caught behinds or lbws that might be contentious."
Yorkshire's Darren Gough said: "There's no Hawkeye, there's no snicokmeter, though there might be when it gets to the semi-finals. It's basically down to the third umpire's eye."