Vikram Solanki made cricket history at 1302 BST on Thursday by becoming the first super substitute as England took on Australia at Headingley.
The batsman replaced Simon Jones, who bowled his 10 overs for 28 runs, and left the field at the end of the 31st over with Australia on 118-4.
Under the new rules, Jones cannot play any more part in the first match of the NatWest Challenge.
Umpire Rudi Koertzen made a "T" signal to announce the substitution.
Jones said afterwards it was not pre-planned who would be substituted but it was clear it would be a tailender.
He told Sky Sports: "I think Steve Harmison's quite jealous that I'm the lucky one.
"I didn't bat well at Lord's when I was yorked so I think I was a prime candidate to be chosen.
"I'll hang around and watch the boys now!"
Australia brought their supersub Brad Hogg on in the 22nd over to replace batsman Matthew Hayden.
Hogg, a spinner, would have been allowed to bowl a full 10 overs if he wanted to because Hayden had not bowled a ball. In the event he bowled six, taking 1-30.
He made an immediate impact by dismissing Andrew Strauss, caught behind for 41, with his third delivery.
In a further twist Hayden stayed on the field of play but as the 12th man after Shane Watson strained his left thigh.