Khan's first fight under Rubio (l) ended in his first defeat in 19 bouts
Amir Khan has fired trainer Jorge Rubio after just one fight as he looks to bounce back from suffering the first defeat of his career.
The Commonwealth lightweight champion lost his unbeaten record when knocked out by Breidis Prescott on 6 September.
A spokesman for Khan's promoter, Frank Warren's Sports Network said: "Jorge has been relieved of his duties."
A new trainer has yet to be confirmed but top American Freddie Roach is thought to be in the frame again.
The decision comes despite Khan's insistence, in the wake of his defeat at Manchester's MEN Arena, that he would stick with Rubio - regardless of the Cuban's fateful decision to choose Prescott as the Bolton fighter's opponent.
The Colombian went into the fight with 17 knockouts from 19 fights and promptly despatched the 2004 Olympic lightweight silver medallist in just 54 seconds.
Khan, 21, sacked Salford trainer Oliver Harrison early this year and then surprisingly announced Rubio as his new coach this summer despite being linked with a host of top names, including Roach.