Chambers is the reigning European indoor 60m champion
Dwain Chambers produced a stunning display to win the 60m final in the fastest time this year at the indoor World Trials and UK Championships.
Chambers, 31, confirmed his prospects as a World Indoor gold medal contender with a time of 6.50 seconds.
Harry Aikines-Aryeetey finshed second with a personal best of 6.55secs with Craig Pickering in third in Sheffield.
Chambers, who won silver at the World Indoors in 2008 in Valencia, is now ranked number one in the world.
"It's nice but it's still early days, I'm sure the Americans are going to come out and do something crazy," said Chambers, who served a two-year suspension for taking performance-enhancing substances, after his victory.
"It's nice to know I have made the team and can hopefully focus my attention towards Doha now."
Chambers will be joined by Aikines-Aryeetey, 21, in the Great Britain and Northern Ireland team for next month's World Indoor Championships in Doha, Bahrain.
"I'm just happy that I'm realising my potential and things are moving in the right direction," said the Londoner.
Olympic relay gold medallist Mark Lewis-Francis finished fourth in a time of 6.67 secs.
Elsewhere, Chris Tomlinson missed out on a seventh long jump title as World Championship finalist Greg Rutherford leaped a season's best of 7.94m, with Tomlinson registering 7.75m.
Elsewhere, Kate Dennison won her fifth successive pole vault title but could not improve her British record following three failures at 4.58m.
Vikki Hubbard missed out on the qualifying standard of 1.92m despite winning the high jump with a clearance of 1.87m, but the Birchfield Harrier can still reach the mark in Croatia next week.
Callum Priestley celebrated his 21st birthday with victory in the 60m hurdles, but failed to make the 7.65secs qualifying standard by 0.04secs, while Joice Maduaka, 36, won her sixth national title in the 60m.
And world bronze medallist Jenny Meadows dominated her 800m heat, leading from start to finish to qualify for Sunday's final.