Chambers was the third fastest qualifier in the final
Dwain Chambers capped his controversial comeback by claiming silver behind Olusoji Fasuba in the 60m final at the World Indoor Championships in Valencia.
Chambers set a new personal best with a time of 6.54 seconds, the same time as former world 100m champion Kim Collins, who also took silver in a dead heat.
"I would like to have won, but I can't complain with a PB," Chambers, who served a two-year drugs ban, said.
Briton Simeon Williamson finished in seventh with a time of 6.63 secs.
Nigerian Fasuba was in imperious form, storming to victory in 6.51 secs, equalling his season's world-leading time.
"I felt great," said the 23-year-old Fasuba. "After the semi-final I strongly believed that I would win the gold."
Inevitably the spotlight in the final was on Chambers - who was suspended after testing positive for the steroid THG in 2003 - on his international return.
If there's a chance of me winning then it's something we will consider
Dwain Chambers on appealing against his lifetime Olympic ban
UK Athletics were reluctant to include Chambers in the squad because of his doping history but had little choice under the rules of their selection policy when he won the trials in February.
The 29-year-old comfortably won his semi-final and was the third fastest qualifier after Fasuba and American Michael Rodgers.
In a tight final, Chambers and Collins of St Kitts and Nevis were inseparable on the line and the pair were both awarded silver.
"I can't please everybody, but I got a medal for my country," said Chambers.
"I wanted to go and win, and I put my self under more pressure than anything. The better man won on the day."
The Londoner had described Friday's final as his "Olympic Games" - but Chambers refused to rule out a High Court appeal against his lifetime Olympic ban.
"I want to check my situation, check if it's worth appealing and if there's a chance of me winning then it's something we will consider," he said.
"But for now I just want to celebrate with my team-mates and go and have some fun."
Chambers received a warm reception from the crowd when he took to the track in the afternoon's heat.
And the Londoner had warmed up with a T-shirt emblazoned with the phrase 'Just say no' to publicise his anti-drugs stance.
"I feel humbled by my experiences over the past couple of years and all I can do is come out and do things the right way in a positive manner and keep smiling all the way through it," he said after the first round.
"This is bringing attention to myself and the sport whether it be good, bad or ugly. I want to use this as an opportunity to get some positive attention to show that taking drugs is wrong and that you can do it clean."