Italy coach John Kirwan admits he is facing an injury crisis ahead of next week's Six Nations clash with France following the 39-7 loss to England.
Fabio Ongaro, Giorgio Intoppa and Matteo Barbini all failed to finish the Wooden Spoon decider at Twickenham.
"Intoppa has a suspected cruciate ligament injury to his right knee and he is definitely out," said Kirwan.
"Ongaro took a knock to the leg. It isn't a fracture but we'll have to look at it." Barbini has a broken thumb.
Meanwhile, flanker David Dal Maso suffered a hyper-extension of his elbow and his fitness to face France will now be reviewed.
Kirwan also admitted that his side must cut out simple errors if they are to turn good performances into victories.
"We are in games but are making mistakes at critical times and that's costing us," he said.
"When we turn up teams respect us and know they have been in a game. The lineout and scrum went well against England but we let ourselves down at the vital moments."