England will be without Steven Gerrard, Owen Hargreaves and Darren Bent for the friendly against Germany on Wednesday.
Hargreaves, Gerrard and Bent will play no part against Germany
Liverpool midfielder Gerrard will rest his broken toe while Manchester United's Hargreaves is still feeling pain from his knee injury.
Bent misses out because of a thigh injury he suffered in Tottenham's win over Derby on Saturday.
England have also confirmed Portsmouth defender Sol Campbell will play no part in the game because of a groin injury.
There was some good news for England coach Steve McClaren on Monday as David Beckham took part in the afternoon training session.
The 32-year-old, who has been struggling to recover from an ankle injury, played his first full game for new club LA Galaxy on Saturday before taking a flight from New York to London.
But he was able to join in training with the other players.
Earlier, former England boss Sir Bobby Robson had expressed concerns about Beckham's ability to combine playing in the US with representing England.
Robson told BBC Sport: "In the short term he looks OK, but the longer he plays in America, with respect to the football being played there, the less competitive he will become.
"It is not the greatest level of football. He will lose the match sharpness he needs to play at the top level with England."
However, McClaren was in positive mood after watching Beckham train.
"I think David can combine playing out there and coming here," he said.
"I understand everyone's concern about that - but the proof will be not just in his performances but also in training.
"When we brought him back (last time) in the Monday training session, there was a lot of pressure on him and a lot of people looking.
"His performance in training got rid of the doubts.
"We will know that. That is what we assess. It is not just in games we look at him, it is in training as well."