David Beckham played his 108th match for England on Wednesday night, but it didn't stop Fabio Capello's side losing 2-0 to Spain.
The defeat to the Euro 2008 champs was the first time England have lost in nine games.
Elsewhere, Northern Ireland moved into the second spot in World Cup Qualifying group three, by beating San Marino 3-0.
And Wales played well, but lost 1-0 to Poland in their match on the Algarve in Portugal.
Craig Bellamy had to be led away after a row with a fan after the match.
Northern Ireland beat San Marino 3-0
The Wales captain got annoyed when he was interrupted while he was doing an interview.
Beckham's appearance in Seville means he has equalled the most number of caps by an England outfield player.
But manager Capello said he thought Beckham would be disappointed to get the record on a night his side lost.
Capello said: "I would have been more happy if we had won, but I am happy for him."
In the mix
Northern Ireland manager Nigel Worthington said his side were "back in the mix" after their win over San Marino.
Goals from Gareth McAuley and substitute Chris Brunt were the highlights of the match.