Glasgow's Museum of Transport at Kelvin Hall closed its doors for the final time on Sunday ready for its move to the Clyde.
The museum said it would be "a huge task" to relocate 13 very large objects and 3,000 slightly smaller ones.
Some of the larger vehicles will have to be moved at night with road closures and police escorts.
The exhibits will be re-housed in the new £74m museum on the banks of the Clyde scheduled to open in spring 2011.
There will be more than twice as many objects on display in the museum's new home, including a large South African train which has recently been on show in George Square.
A total of £69m of the new development has been funded by Glasgow City Council and The Heritage Lottery Fund and the remaining £5m is being raised by The Riverside Museum Appeal.
They have raised about £3m so far and are appealing to businesses, trusts and individuals to help raise the remaining £2m.
Museum manager Lawrence Fitzgerald explained that one of the problems with the old building was that it was very hard to use audio-visual installations without digging up the floor to install power points. He said: "It was not a very flexible building."
He added: "With the new museum we want to tell people's stories, the stories behind the objects, so there's going to be a lot of audio-visual oral histories and so on."
Among the new exhibits will be a scooter loaned from Franz Ferdinand's Alex Kapranos, a car from Robby Coltrane and the Subaru Impreza in which Colin McRae won the world rally championship.
To bid farewell to 22 years at the old museum in the west end there were a series of free events over the weekend, including music and dance performances, historic bus tours, a 'speedy racers' competition and a closing ceremony.