The Museum of Transport in Glasgow is to close its doors to start moving exhibits to its multi-million pound replacement.
The free-to-enter visitor attraction at Kelvin Hall will shut for good on 18 April.
A £74m Riverside Museum is being built to accommodate the exhibits, but it is not due to open until spring 2011.
A number of small displays have already been removed from Kelvin Hall which has been the museum's home for 22 years.
Before it closes in April, there will be a fortnight of free events and a special closing ceremony.
The museum has proved a popular attraction with an average 500,000 visitors annually.
The bulk of funding, £69m, has already been committed by Glasgow City Council and the Heritage Lottery Fund.
But millions of pounds are still needed and the public is being asked to help with fundraising for the new purpose-built museum overlooking the Clyde.
Bailie Liz Cameron, head of Culture and Sport Glasgow, said: "For many, the occasion will be one tinged with a little sadness amongst the great hope and expectation that is building as the magnificent Riverside Museum takes shape nearby.
"The new museum will be home to many of the much loved exhibits housed in an iconic building with space for many new attractions which will educate and captivate visitors for generations to come."