Aberdeenshire Council has said about 4,000 pupils will be affected by a strike by Stagecoach staff.
Highland Council said services will not operate as usual on routes to 18 of its schools.
Previously, when industrial action was planned, Aberdeenshire Council asked parents to car-share where possible to avoid congestion at school gates.
Services to the following schools and colleges are affected:
ABERDEENSHIRE
Aberdeen College.
Banff and Buchan College.
Buchanhaven Primary School.
Dales Park Primary School.
Fraserburgh Academy.
Mintlaw Academy.
Mintlaw Primary School.
Peterhead Academy.
St Fergus Primary School.
Tyrie Primary School.
Banff Academy.
Bracoden Primary School.
Fintry Primary School.
Macduff Primary School,
Markethill Primary School.
Portsoy Primary School.
Rothienorman Primary School.
Turriff Academy.
Markethill Primary School.
Whitehills Primary School.
Alford Academy.
Ellon Academy.
Ellon Primary School.
Forgue Primary School.
The Gordon School Academy.
Inverurie Academy.
Kemnay Academy.
Kinellar Primary School.
Kintore Primary School.
Meldrum Primary.
Meldrum Academy.
Cults Academy.
Aboyne Academy.
Aboyne Primary School.
Banchory Academy.
Banchory Primary School.
Catterline Primary School.
Crathes Primary School,
Luthermuir Primary School.
Mackie Academy.
Mearns Academy.
Strachan Primary School.
Durris Primary School.
Lairhillock Primary School.
Portlethen Primary School.
Portlethen Academy.
HIGHLAND
Stagecoach buses will not be operating on routes to the following schools:
Resolis Primary School.
Fortrose Academy (feeder buses).
Dingwall Academy.
Alness Academy.
Invergordon Academy.
Tain Royal Academy.
Charleston Academy.
Culloden Academy.
Millburn Academy.
Glen Urquhart High School.
Glenurquhart Primary School.
Kilchuimen Academy.
Bun Sgoil Ghaidlaig.
Inshes Primary School.
Balnain Primary School.
Auldearn School.
Rosebank School.
Millbank School.
The Invergarry to Lochaber High School bus which is operated from the Inverness depot was expected to operate as normal.
The Lochinver to Ullapool High School service and those in Badenoch and Strathspey area were also due to run.
All services are expected to resume as normal on Wednesday.
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