The accident happened on the A161 between Low Burnham and Haxey on Thursday afternoon.
The woman who died, a 35-year-old from Haxey, was driving a Peugeot 307 which collided with a BMW 118 driven by a 40-year-old woman from Belton.
The BMW driver suffered broken arms and legs and is in a serious but stable condition at hospital in Scunthorpe.
Two girls, aged 13 and 11, who were travelling with her escaped with minor injuries.
The road between Epworth and Haxey was closed for more than six hours to allow the scene to be examined and the vehicles to be recovered.
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