A severe flood warning is in place at a number of villages along the River Great Ouse in Bucks and Beds.
Parts of Radwell and Milton Ernest are underwater, while Harrold, Chellington, Felmersham, Odell, Oakley, and Pavenham are also affected.
About 70 homes and businesses were flooded when the River Great Ouse burst its banks in Buckingham on Saturday.
Residents were warned to steer clear of the water which could be contaminated with chemicals and sewage.
Bedfordshire Police closed five bridges over the River Great Ouse and set up a refuge centre at a school in the village of Turvey.
The Buckinghamshire Fire and Rescue Service said it handled about 55 calls to flood incidents across the county.
In the early hours of Saturday, the river's water level became higher than in 1998, when the town last experienced serious flooding.
Residents forced to leave their homes spent the night in the town's Radcliffe Centre.
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