Karachi has seen the biggest pro-Taleban protests
It has closed the Taleban consulate in Karachi, which President Pervez Musharraf said was "not serving any purpose" and was "having negative effects".
On Tuesday the Taleban ambassador in Islamabad, Abdul Salam Zaeef, was told not to use his press briefings, which have been broadcast around the world, to criticise foreign governments.
Meanwhile a second prominent Islamic leader supporting the Taleban, Fazlur Rehman, has been taken from his home and placed under detention in a government rest house, his supporters say.
End of the week
The Taleban have been until the end of the week to close their Karachi consulate.
Pakistan Foreign Ministry spokesman Aziz Ahmed Khan told journalists that the consulate staff "have to be withdrawn, so naturally they have to go back to Afghanistan".
Karachi is Pakistan's biggest city and main commercial centre. It has also seen the biggest pro-Taleban demonstrations in the country since America began its military actions against Afghanistan last month.
The restrictions on the Taleban coincide with Pakistani President Pervez Musharraf's first absence from the country since the 11 September attacks on the United States.
Pakistan is the only country that still recognises the Taleban.
But President Musharraf has outraged a substantial minority of Pakistanis with his support for the US action against the Taleban which is aimed to overthrow the movement.
Analyst Khalid Mahmood told the AFP news agency: "The government does not want to convey the impression that it is not firmly in control of the agitators. The closing of the Taliban consulate in Karachi and restrictions on the Afghan ambassador give a clear message that the government wants to show that it is in control."
The embassy in Islamabad, along with the Taleban consulates in the towns of Quetta and Peshawar close to the Afghan border, still remain open.
No contact
Fazlur Rehman, a vocal ally of the Taleban and leader of the Jamiat Ulema-e-Islami (JUI) party, has been taken from his home, where he was under house arrest, to a government rest house, party members told Reuters news agency.
"He was taken away from his house on Wednesday because authorities said he continued to make statements against the government despite being under house arrest," party member Abdul Jalil Jan said.
"We have no contact with him now."
Last month Mr Rehman was charged with treason after calling on his followers to attack members of Pakistan's armed forces.
Sedition
On Tuesday, Qazi Hussain Ahmad, who heads Pakistan's main Islamic party, was moved from his home, where he has been under house arrest, to a government rest house.
Mr Ahmad, leader of the Jamaat-e-Islami party, said the rest-house has been converted into a jail and that he would be unable to contact anyone from there.
He was charged with sedition after urging the army to overthrow President Pervez Musharraf.
Police say they are taking extra measures to ensure there is no trouble on Friday when Islamic parties have called a nationwide strike to protest against the US campaign in Afghanistan.
Police are reported to have drawn up a list of suspected trouble makers who could face arrest.