Pricey Polo (for her):
Chinos: $59-75
Shirts: $59-140
Sweaters: $98-325
Navy blazer: $398
The workers said they have been compelled to spend almost half their annual salary on the latest Polo fashion simply to keep their jobs.
New staff would receive wages in advance to pay for the clothes, according to the lawyer representing the shop workers, Patrick Kitchin.
Mr Kitchin described the shop assistants as "captive customers".
"Polo has created an unfair advantage against other employers by wardrobing," he said.
Refund
Polo shop assistant Toni Young filed the complaint on behalf of herself and some of her colleagues after having spent between $8,000 (£5,145) and $10,000 a year on Polo fashion since she joined the company in 1997.
Pricey Polo (for him):
Chinos: $58-79
Shirts: $49-97
Sweaters: $69-397
Navy blazer: $795-850
Ms Young, who worked in Polo's San Francisco shop, earned a salary of about $22,000, Mr Kitchin said.
Ms Young wants the company to reimburse her for the money she spent on Polo fashion, and she wants the firm to abolish its uniform policy.
Polo Ralph Lauren declined to comment on the pending litigation.