Saudi Foreign Minister Prince Saud al-Faisal blamed the rhetoric at the 10 July briefing - which US officials have publicly disavowed - on elements within the Bush administration.
"Unfortunately, there are people in certain departments who try to raise doubts and shake the strong historical ties between our two countries," Prince Saud said on Wednesday in a statement carried in Saudi Arabia's state media.
"I am confident that they will not succeed. The Saudi-American relationship of friendship and alliance that goes back 60 years is excellent in all fields," he said.
The Pentagon has been trying to distance itself from the briefing, given by an analyst with the Rand Corporation think tank, in which the Saudis were accused of funding terrorism.
According to the Washington Post newspaper, a Rand analyst told a Pentagon advisory body that the US should punish Saudi Arabia if it failed to stop supporting Islamic extremism.
But the US Defence Secretary, Donald Rumsfeld, has said the briefing did not represent the government's or the Pentagon advisory board's views.
New tensions
Despite the insistence from both countries that they are long-standing allies, there are new tensions between Washington and Riyadh, especially over possible US plans to attack Iraq.
In an interview with the Associated Press on Wednesday, Prince Saud reiterated that Washington will not be allowed to use Saudi soil for such an attack.
"We have told them we don't [want] them to use Saudi grounds," the foreign minister said.
"We are against any attack on Iraq because we believe it is not needed, especially now that Iraq is moving to implement United Nations resolutions," he added, referring to recent overtures from Iraq to discuss arms inspections.
The US suspects Baghdad of trying to develop chemical, biological and nuclear weapons, and has pressed for UN weapons inspections to resume.
Iraq has recently offered to hold technical talks on arms inspections.