There are calls for urgent improvements to the A454 road between Menai Bridge and Beaumaris after it was closed by fallen trees again.
The road goes through a heavily wooded area on Anglesey
Anglesey councillor Eurfryn Davies said the latest 20-hour closure affected both local people and businesses.
Hotel proprietor Mary Brain said she was fed up with the situation which affected her trade each time.
A council spokesman said it would cost £250,000 for a feasibility study to look into a new road scheme.
"This time the road was first closed just on one side yesterday morning and then late afternoon it was closed altogether," said Mr Davies.
"There has been talk of two different routes for a new road between Menai Bridge and Beaumaris, but I think the best, and cheapest, option would be to improve the present road, getting rid of the corners, and cutting back the trees in the process," he said.
"The problem now is that the trees grow at an angle and improving the road would make the area much safer."
The present main road which hugs the island along the Menai Strait was built in 1912, with improvements in the 1960s, said Mr Davies.
"The frequent closures do not add much time-wise to the journey between the two places, but it affects many, many people not just in Beaumaris but in Llanddona, Llangoed, and Beaumaris.
"There are two alternatives to the main road, but both are narrow lanes, with barely enough room for vehicles to pass," he added.
Mary Brain, proprietor of the Gazelle Hotel, said her trade was affected each time the road was closed.
"I'm fed up," she said. "It has happened several times now.
"What we find is that no-one tells us and we never know which side the road is closed - either between us and Menai Bridge or between us and Beaumaris.
"It has got to the stage now when people are asking whether it is safe to travel along this stretch.
"The trees should be cut down or at least trimmed right back. I'm worried someone will be killed."
An Anglesey Council spokesperson said plans for a new link road between the A55 and Beaumaris had been considered by the authority.
"A full feasibility study, however, would cost somewhere in the region of £250,000.
"Funding, by way of the assembly's transport grant, would be required to undertake this kind of in-depth study."