The Stirling Smith is to host a unique exhibition featuring works by Leonardo da Vinci to mark the Prince of Wales' 60th birthday later this year.
The sketch of 'two grotesque profiles' dates from 1485-90
The museum is the only Scottish venue which will take part in the exhibition.
Ten of the Royal Collection's finest drawings by the Renaissance master will appear at four galleries across the UK.
The drawings, which cannot be on permanent display because of potential light damage, are considered among the most important by the artist.
The works are usually housed in the Royal Library at Windsor Castle.
The Smith's chairman of trustees, Cllr Colin O'Brien, said that despite being a "special opportunity" for the gallery, the exhibition would also prove a challenge.
He added: "This will be an expensive project - the additional security required is a huge cost in itself - and we are looking for financial assistance.
"The Smith hopes for the support of the Stirling business community and the public to make this exhibition happen.
"We need widespread financial aid, of any amount, and at least one major sponsor for this very special exhibition.
"This could also provide an opportunity for businesses to be more involved with the Stirling Smith on a regular basis."
The exhibition will begin in Cornwall in May before moving to the Smith Art Gallery and Museum on 8 August, where it will run until 2 November.
It will then move on to the National Library of Wales before concluding at the Manchester Art Gallery in May 2009.