Tests for the bluetongue disease have been carried out on a cow at a farm near Dumfries.
The alarm was raised by a vet who saw suspicious symptoms in the animal before sending samples for analysis.
However, the results of the test came back negative. The name of the farm involved has not been released.
The first UK case of bluetongue, which is spread by midges, was confirmed in East Anglia in September. It has since been found elsewhere in England.