Campaigners are calling for a public inquiry into plans to build large chicken sheds near West Linton.
Plans for the large poultry farm have caused opposition
The first of 10 proposed sheds has been approved after the Scottish Executive overturned councillors' calls for an Environmental Impact Assessment.
Glenrath Farms Ltd wants to build the sheds, each housing 32,000 birds, on the site near Blythbank.
Protesters say they will continue to fight the plans for what would be Europe's largest egg farm.
The company has promised to address concerns about traffic and pollution.
The unit would be situated in a dip in the landscape and painted to blend in with its surroundings.
However, Doreen Graham, of Blythbank Action Group, said they would still oppose the scheme.
"We were advised by the Scottish Executive that under planning regulations if a planning application was known to be part of a larger development an EIA could be requested," she said.
"They actually went back on the advice given to the group."
The executive has said that the Environmental Impact Assessment is not required for the first structure because it would not have a significant impact on the area.