Deer are involved in about 10,000 road accidents, the commission said
Motorists should expect higher numbers of deer on the roads, the Deer Commission for Scotland has warned.
It said pregnant females were pushing last year's young off their territory. The activity will run until mid-May.
Spokesman Jamie Hammond said drivers should be prepared for the animals suddenly running onto the road.
He said there were about 10,000 traffic accidents involving deer a year that lead to, on average, two to three deaths of drivers or passengers.
The Stirling-based commission and Transport Scotland will run 65 warnings along routes in the north east of Glasgow, the Central Belt, Aberdeen, Dundee, parts of Morayshire and the A9 to alert drivers of the dangers.
Mr Hammond said: "It is certainly a serious problem and one that is increasing in certain areas.
"We often find that people who live in and around towns think these warnings aren't relevant to them, but in fact accident rates are often higher in areas with more people and more cars on the road.
"Accidents certainly don't only occur on rural roads in the Highlands. We urge drivers to be on the lookout for deer on the sides of roads or emerging from bushes or trees on road-sides.
"If you're driving by wooded areas or areas where you've seen deer before, slow down."