The mill employs 40 staff and provides work for 130 weavers
The owner of a mill which produced 95% of all Harris Tweed has ceased production for the rest of the year.
Brian Haggas told the BBC he would make no more tweed until the end of the year, depending on demand.
The decision raises doubts about the future of his remaining 40 staff and 130 weavers who depended on the mill on Lewis for their livelihood.
The weavers said the situation had never been so bleak and fear many of them will have to leave the industry.
Cloth produced at the former Kenneth Mackenzie mill has gone into making tweed jackets.
With close on 70,000 jackets now ready, Mr Haggas said he would not weave any more cloth until the end of the year.
Meanwhile, two smaller mills on Lewis which have been revived are not yet in a position to keep 130 home-based weavers in work.