The line passes through picturesque locations including Glenfinnan station
The West Highland line has been voted the top rail journey in the world by readers of independent travel magazine, Wanderlust.
It came ahead of two trips in Peru and the Trans Siberian Express.
The line from Glasgow to Mallaig is a 164 miles long and runs by Loch Long, Loch Lomond and Loch Eil.
It crosses the world's first concrete rail viaduct at Glenfinnan, which was featured in the Harry Potter films, and passes the silver sands of Morar.
Passengers can also see Britain's most westerly station at Arisaig.
The line has also featured in programmes such as the BBC's Great Railway Journeys of the world.
The West Highland line beat off competition from 400 other nominated rail journeys from around the world.
The awards attracted almost 3,000 readers' votes.
Wanderlust editor-in-chief Lyn Hughes said: "We have had a terrific response and it is obvious that the appetite for rail travel is ever increasing.
"Having a Scottish winner for this award is particularly exciting, and shows you don't have to travel far for truly world-beating scenery."
Steve Montgomery, acting managing director of ScotRail, said: "We are delighted that Wanderlust readers, who are so passionate about travel, have chosen the West Highland Line.
"It is a testament to our staff, our services - and the line, now the world's top rail journey.
"We look forward to welcoming more visitors to savour the line's delights."