Spirits like can only legally be sold in 25ml or 35ml quantities
Licensees in Fife have been warned that checks will be carried out throughout August on the size of their measures.
Trading Standards officers will be conducting random spot checks to make sure pubs are selling accurate sized spirits to customers.
A similar survey in Fife last year found only one in 10 licensed premises were selling accurate measures and more than half offered short measures.
But more than a third of those tested were found to be over measures.
Under the Weights and Measures Act 1985, gin, rum, vodka and whisky can only be sold in quantities of 25ml or 35 ml.
It also says that notices must be displayed stating which quantity the pub is selling as a "shot.".
In October 2007, officers tested a total of 72 spirit samples, only seven of which were accurate.
Alan Hamilton from Fife Council said: "It is in everyone's interest that accurate measures are sold in Fife, particularly during the summer months when there are so many visitors to local pubs and restaurants.
"One of the aims of the project is to advise and educate licensees, however, we will issue a written warning if measures are found to be inaccurate.
"Any measures that are seriously deficient, may result in a report being submitted to the procurator fiscal."
Trading standards officers will carry out random checks next month but will also revisit some of the premises that received a written warning last year.