The number of people confirmed with E.coli O157 in the Dunfermline outbreak has increased to 11.
The cases are linked to the Careshare nursery
Two new cases linked to the Careshare nursery at Lauder College have been confirmed, both of whom are adults related to infected children.
A spokesmen for NHS Fife said they are both well and the number of possible further cases has fallen to 22, with 60 people being given the all clear
Four toddlers remain in hospital, the condition of two of them is improving.
Dr Neil Hamlet, consultant in public health medicine at NHS Fife, said: "There have been no further hospital admissions related to this outbreak since 12 May."
He added: "Preventing spread of E.coli O157 infection between people depends on effective handwashing after using the toilet, before and after handling food, and before eating. This needs to be supervised for younger children."