Almost 450 members of the RMT voted in favour for strike action
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Talks aimed at averting a rail signalling strike will resume on Monday.
Almost 450 members of the Rail Maritime and Transport (RMT) union plan to strike from midday on Tuesday 7 October and again on Thursday 9 October.
Network Rail said their request for the strike to be suspended was refused by the union bosses.
It comes after a day of negotiations in Glasgow, hosted by the conciliation service Acas.
Union chiefs met with Network Rail managers on Friday to discuss the row over rosters and transfers.
Its members have voted for a ban on overtime and rest-day working, blaming a breakdown in industrial relations.
On Thursday Liberal Democrat leader Tavish Scott asked First Minister Alex Salmond to intervene in the dispute, saying thousands of passengers would be hit by four days of disruption.
But Mr Salmond said the Scottish Government had "no plans" to step in.
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