The proposed route of a 400,000 volt overhead transmission line, stretching 137 miles (220km), has been published by Scottish and Southern Energy.
The new pylons will carry power from new wind and water schemes
The new £320m route follows 18 months of consultation after the original plans sparked objections.
The line will consist of about 600 pylons, some up to 213ft (65m) high, which will take power from Beauly, near Inverness, to Denny, near Falkirk.
Part of the proposed line will run through the Cairngorms National Park.
Scottish and Southern Energy said that there would be fewer pylons and less transmission lines than currently run through the park area.
There will be 76 pylons compared to 128 at present.
The company also said that overall there would be 200 fewer pylons than on the existing 132,000 volt transmission line, but that they would be bigger.
The height of the new pylons will range from 42m to 65m.
Almost 80% of the pylons will be lower than 57m. The height of the pylons on the existing line ranges from 25m to 41m.
Campaigners had urged the company to put the cable underground but Scottish and Southern said this would could cost between six and 12 times as much.
The power firm said that about 60% of the line would be built on a route adjacent to the existing line, which it will replace.
The upgraded pylon line will carry power produced by new wind farms and hydro schemes in the Highlands and islands to customers further south.
A spokesman for Scottish and Southern Energy said that the new route was published on its website.
He said that anyone concerned about where the line would go should consult the online map.
An application to build and operate the line would soon be submitted to Scottish Executive ministers, the company said.
Spokesman for protest group Stirling Before Pylons, Peter Pearson, said: "We are massively disappointed to learn there have been no significant changes to the proposed route.
"The proposed line passes almost directly over some houses, and near to many others."
He pointed out some of the areas most badly hit included Dunblane, Braco, Kinbuck, Ashfield, Sheriffmuir, Logie, and Plean.
Mr Pearson added the pylons were almost as high as Scotland's national landmark, the Wallace Monument, and threatened to come within a few hundreds yards of it.
He added they would be an eyesore from Stirling Castle and right across the Forth Valley and the structures would damage a "tranquil and sensitive" area.
The protest group called on Stirling Council to object to the "poorly thought out" proposals and demanded a public inquiry into the plans.