The number of East Midlands seats dropped from six to five
The UK Independence Party lost a seat in the European elections in the East Midlands, the only English region where it ended the night having lost an MEP.
A 9% fall in UKIP's share of the East Midlands vote saw it win one seat, down from two in the 2004 elections.
The Conservatives held on to their two seats with a win for Emma McClarkin and the re-election of Roger Helmer.
Labour's Glenis Willmott and Liberal Democrat Bill Newton-Dunn were re-elected, as was UKIP's Derek Clark.
The enlargement of the European Union has reduced the number of East Midlands seats from six to five.
Ms McClarkin - former press officer to Mr Helmer - has been elected for the first time.
"I just think it's fantastic. I'm just so excited," Ms McClarkin said.
"It's a huge privilege to be in this position."
Voter turnout in the region fell from 43.7% to 37.34%.
On a night when Labour performed badly across the UK, the party lost votes in the East Midlands and saw its share of the vote fall from 21% to 16.9%.
But a larger slump for UKIP saw Labour jump from third in 2004 to second behind the Conservatives this year.
Ms Willmott said: "We've actually done better in the East Midlands than we did last time.
"Instead of coming third we've come second."
She called on the party to rally behind the prime minister.