More than 300 second-hand bikes are being sent from Hullavington in Wiltshire to the Gambia.
They will be sent as part of the Bikes 4 Africa project - the idea of the South Cerney-based charity Jole Rider.
The bikes will be used by children in the Gambia who often have to walk 10 miles to school every day.
The charity said it is desperate to raise more cash to pay for shipping as it costs about £3,000 per container of about 300 bicycles.
The Jole Rider charity also helps form links between UK secondary schools and schools in Africa, enabling UK students to travel there for a seven or 10-day programme.
To date the charity has sent 600 bikes to Africa.