Around 150 peace campaigners gathered at an US air base in Suffolk to demand the removal of nuclear weapons.
The rally comes one month after a Mildenhall protest was stood down
The Campaign for Nuclear Disarmament claims there are more than 100 American nuclear bombs at RAF Lakenheath.
"There are B61 bombs stored at the base which are ten times more powerful than the Hiroshima bomb," CND Vice-President Bruce Kent said.
The USAF neither confirms nor denies the existence of tactical nuclear bombs being stored at Lakenheath.
"Their existence is an obstacle to nuclear disarmament which is the issue in front of us," Bruce Kent said.
"Hundreds of US nuclear weapons are kept on bases throughout Europe as part of Nato's security strategy," he added.
Chairman Kate Hudson said: "Nato is a cold war relic which should have been disbanded when the Warsaw Pact was dissolved.
A spokesman for Suffolk Police said the demonstration was peaceful with officers experiencing no problems at all.
The protest comes one month after a peace camp outside RAF Mildenhall, also in Suffolk, was stood down.
Campaigners spent two weeks protesting against US flights landing there to refuel on their way to Israel.