A two-year-old child has become the second suspected case of E.coli from a Brighton nursery since it was closed following an outbreak of the bacterium.
The nursery at the hospital cares for children of NHS staff
The Hilltop Nursery was closed on Friday after a three-year-old caught the infection and an 18-month-old was treated for a suspected case.
The three-year-old is being treated in hospital. Those suspected of having E.coli are being cared for at home.
A spokeswoman said the nursery would reopen when it was clear of the bug.
The nursery caters for children of NHS staff working at Brighton General Hospital and is on the hospital site.
The spokeswoman for the South Downs Health NHS Trust said the three-year-old, who is in hospital in London, was said to be improving.
In a statement she added that the nursery was being deep cleaned in line with national guidelines on preventing the spread of infection.
"The nursery will reopen when sufficient nursery staff test clear of any sign of the infection, but not before Monday 18 September," the statement added.
People who contract the infection usually recover in about two weeks.
Forty children aged from six months to school age are registered at the nursery.